Rubisco核酮糖二磷酸羧化酶
PRODUCT INFORMATION
background Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.Source of Rubisco standard: Rubisco protein was purified directly from a plant tissue - spinach.
antibody format
does not apply
quantity
100 µl, lyophilized, for reconstitution add 90 µl of milliQ water.
Please notice that this product contains 10% glycerol and might appear as liquid but is provided lyophilized.
storage store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
tested applications western blot (WB)
related products Primary antibodies matching Rubisco standard are:
AS01 017 | anti-RbcL Type I, chicken global antibody AS03 037 | anti-RbcL Type I and II, rabbit global antibody
collection of other protein standardscollection of matching global antibodies
additional information The RbcL protein standard can be used in a combination with Agrisera global antibiodies (AS01 017from chicken or AS03 037 from a rabbit) to quantitate RbcL from a wide range of species. Global antibodies are raised against highly conserved amino acid sequence. This standard is also included in following kits: Educational antibody kit - photosynthesis,Rubico quantitation kit,
Quantitative western blot: detailed method description, video tutorial
APPLICATION INFORMATION
recommended dilution Standard curve: 3 loads are recommended (0.5, 2 and 4μl).
For most applications a sample load of 0.2 μg of chlorophyll/well will give a RbcL signal in this range.
Positive control: a 2 μl load per well is optimal for most chemiluminescent detection systems. Higher standard concentration needs to be used to allow detection by Coomasie stains. Such gels with higher standard concentration can not be used for quantitation using chemiluminescence.
This standard is stabilized and ready and does not require heating before loading on the gel.
expected | apparent MW 52.7 kDa
additional information Concentration: after re-constitution with sterile milliQ water final concentration of the standard is 0.15 pmoles/µl
Protein standard buffer composition: Glycerol 10%, Tris Base 141 mM, Tris HCl 106 mM, LDS 2%, EDTA 0.51 mM, SERVA® Blue G250 0.22 mM, Phenol Red 0.175 mM, pH 8.5, 0.1mg/ml PefaBloc protease inhibitor (Roche), 50 mM DTT.
This standard is ready-to-load and does not require any additions or heating.
Please, use the 55 kDa size of RbcL for calculations. The pmoles in the standard refer to pmoles of rbcL monomers.
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